2021-4-30 · barcodes. Phusion Plus DNA Polymerase generates PCR amplicons with very few errors due to its extremely high fidelity >100x that of Taq enzyme. Figure 3. Highly sensitive and specific PCR. A 0.5 kb target can be detected from as little as 0.08 ng of human gDNA using Phusion Plus DNA Polymerase. Figure 5. Enzyme tolerance to common PCR inhibitors.
Thermo Scientific Phusion High-Fidelity DNA Polymerases set a gold standard for high performance PCR. Featuring an error rate 50-fold lower than that of Taq and 6-fold lower than that of Pfu Phusion High-Fidelity DNA Polymerase is excellent choice for cloning and other applications requiring high fidelity.
PCR Protocol for Taq DNA Polymerase with Standard Taq Buffer (M0273). Protocols.io also provides an interactive version of this protocol where you can discover and share optimizations with the research community.. Overview. PCR The Polymerase Chain Reaction (PCR) is a powerful and sensitive technique for DNA amplification (1).
2011-3-17 · 2. Phusion DNA Phusion DNA lacI Phusion DNATaq DNA50 Pfu DNA6
We generally recommend using Phusion DNA Polymerase at a concentration of 20 units/ml (1.0 units/50 μl reaction). However the optimal concentration of Phusion DNA Polymerase may vary from 10–40 units/ml (0.5–2 units/50 μl reaction) depending on amplicon length and difficulty.
2020-8-12 · Phusion Hot Start II DNA Polymerase has the ability to stabilize primer-template hybridization. Note that the optimal annealing temperature for Phusion Hot Start II DNA Polymerase may differ significantly from that of Taq-based polymerases. Always use the Tm calculator and
2017-3-22 · The processivity of Phusion DNA Polymerases is approximately 10-fold greater than that of PfuDNA polymerase and twice that of TaqDNA polymerase. This high processivity results in shorter extension times more robust amplification and the ability to amplify long templates (up to 20 kb) in a fraction of the time.
The Phusion High-Fidelity PCR Kit contains a sufficient supply of Phusion High-Fidelity DNA Polymerase Phusion HF and GC Buffers deoxynucleotides magnesium chloride DMSO and DNA size standard to perform 50 reactions (small) or 200 reactions (large). Control template and primers are provided for 20 control reactions.
2020-8-14 · Phusion DNA Polymerase in Phusion HF Buffer is determined to be 4.4 x 10-7 which is approximately 50-fold lower than that of Thermus aquaticus DNA polymerase and 6-fold lower than that of Pyrococcus furiosus DNA polymerase. Phusion DNA Polymerase possesses the following activities 5´→3´ DNA polymerase activity and 3´→5´ exonuclease
Thermo Scientific Phusion High-Fidelity DNA Polymerases set a gold standard for high performance PCR. Featuring an error rate 50-fold lower than that of Taq and 6-fold lower than that of Pfu Phusion High-Fidelity DNA Polymerase is excellent choice for cloning and other applications requiring high fidelity. Phusion DNA Polymerases offer robust performance with short protocol times even in the presence
2013-2-13 · A 2x supermix is now available containing either HF buffer or GC buffer dNTPs and Phusion polymerase. See references section below for links. Thermocycling conditions. 15-30 s/kb extension time. 98C for denaturation. Anneal at 3C above the lowest Tm if the primers are longer than 20nt else at the Tm.
PCR Protocol for Taq DNA Polymerase with Standard Taq Buffer (M0273). Protocols.io also provides an interactive version of this protocol where you can discover and share optimizations with the research community.. Overview. PCR The Polymerase Chain Reaction (PCR) is a powerful and sensitive technique for DNA amplification (1).
2013-2-13 · A 2x supermix is now available containing either HF buffer or GC buffer dNTPs and Phusion polymerase. See references section below for links. Thermocycling conditions. 15-30 s/kb extension time. 98C for denaturation. Anneal at 3C above the lowest Tm if the primers are longer than 20nt else at the Tm.
Phusion DNA 5x Phusion HF 5x Phusion GC DMSO 50 mM MgCl2 . Phusion HF Phusion GC 1x 1.5 mM MgCl2 . . 500U (2U/µL) . 2 U/µL. Fidelity (vs. Taq) 52 X.
2020-8-14 · Phusion DNA Polymerase in Phusion HF Buffer is determined to be 4.4 x 10-7 which is approximately 50-fold lower than that of Thermus aquaticus DNA polymerase and 6-fold lower than that of Pyrococcus furiosus DNA polymerase. Phusion DNA Polymerase possesses the following activities 5´→3´ DNA polymerase activity and 3´→5´ exonuclease
It is a Pyrococcus-like proofreading DNA polymerase fused to a processivity-enhancing domain enabling Phusion Plus DNA Polymerase to generate PCR sequences with high accuracy sensitivity and inhibitor tolerance. • High fidelity —provides >100X higher sequence accuracy than Taq polymerase. • Convenient setup —enables a universal
It is a Pyrococcus-like proofreading DNA polymerase fused to a processivity-enhancing domain enabling Phusion Plus DNA Polymerase to generate PCR sequences with high accuracy sensitivity and inhibitor tolerance. • High fidelity —provides >100X higher sequence accuracy than Taq polymerase. • Convenient setup —enables a universal
2017-3-22 · The Thermo Scientific ™ Phusion Green format is a combination of Phusion DNA Polymerases and 5X Green Reaction Buffer. The buffer includes a density reagent and two tracking dyes (blue and yellow) for direct loading of PCR products on gels. The green buffer does not interfere with the performance of Phusion DNA Polymerases and
It is a Pyrococcus-like proofreading DNA polymerase fused to a processivity-enhancing domain enabling Phusion Plus DNA Polymerase to generate PCR sequences with high accuracy sensitivity and inhibitor tolerance. • High fidelity —provides >100X higher sequence accuracy than Taq polymerase. • Convenient setup —enables a universal
Thermo Scientific Phusion DNA PCR "" Phusion DNA Taq 50 Pfu 6 DNA Phusion DNA
DNA Polymerase Selection Chart. The following table lists properties that should be considered when choosing a polymerase. Since these properties can depend on reaction conditions the primary references should be consulted prior to use in a given application. PCR
2016-10-13 · Taq DNA Polymerase (#EP0402) for example. Incubate purified PCR product with 1x Taq buffer 2.5 mM MgCl 2 0.2 mM dATP and 1 U Taq DNA polymerase in 10 µL reaction mixture up to 30 min at 72 °C. Before adding the overhangs it is very important to remove all the Phusion DNA Polymerase by carefully purifying the PCR product for
2021-4-30 · barcodes. Phusion Plus DNA Polymerase generates PCR amplicons with very few errors due to its extremely high fidelity >100x that of Taq enzyme. Figure 3. Highly sensitive and specific PCR. A 0.5 kb target can be detected from as little as 0.08 ng of human gDNA using Phusion Plus DNA Polymerase. Figure 5. Enzyme tolerance to common PCR inhibitors.
2021-7-21 · Phusion DNA Polymerase . Pfu DNADNASso7d 1. DNA DNAPfu DNATaq DNA102 . 2. DNA
2021-4-30 · barcodes. Phusion Plus DNA Polymerase generates PCR amplicons with very few errors due to its extremely high fidelity >100x that of Taq enzyme. Figure 3. Highly sensitive and specific PCR. A 0.5 kb target can be detected from as little as 0.08 ng of human gDNA using Phusion Plus DNA Polymerase. Figure 5. Enzyme tolerance to common PCR inhibitors.
2018-9-15 · AP231. Yes. Ultra-High Fidelity. 108x. 2-6 kb/min. 15 kb / 20 kb. 1.44 . Platinum® Taq DNA Polymerase HiFi (Life) 11304029.
It is a Pyrococcus-like proofreading DNA polymerase fused to a processivity-enhancing domain enabling Phusion Plus DNA Polymerase to generate PCR sequences with high accuracy sensitivity and inhibitor tolerance. • High fidelity —provides >100X higher sequence accuracy than Taq polymerase. • Convenient setup —enables a universal
Phusion DNA Polymerases offer robust performance with short protocol times even in the presence of PCR inhibitors and generate higher yields with lower enzyme amounts than other DNA polymerase. Phusion High-Fidelity PCR Master Mix is convenient 2X 2.
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